Currently Available Tissue Microarrays

The CHTN distributes TMAs of its own design and from allied organizations. The TMAs listed below with the “CDP” annotation were designed by the Cancer Diagnosis Program of the National Cancer Institute. The application process is slightly different for CDP TMAs than for CHTN TMAs, and the fees are more significant to reflect the larger number of cases, the greater degree of annotated data and the statistical design process associated with these resources. Instructions on how to request TMA slides are found here.

Custom tissue microarray construction is not available as a CHTN service. However, the CHTN Mid-Atlantic Division is able to offer construction of TMAs utilizing specimen blocks provided by the Investigator on a fee-for-service basis in partnership with the UVA Biorepository & Tissue Research Facility. Please contact the CHTN Mid-Atlantic Division for further information.

Cooperative Human Tissue Network (CHTN) Cancer Tissue Microarrays

Cooperative Human Tissue Network (CHTN) Normal and Test Tissue Microarrays

Cancer Diagnosis Program (CDP) Tissue Microarrays

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Cooperative Human Tissue Network (CHTN) Cancer Tissue Microarrays

  1. CHTN_CRC2 - Colorectal Carcinoma Progression

    Description:

    The CHTN_CRC2 TMA contains up to 20 cases of non-neoplastic colonic mucosa, 14 cases of adenomatous polyps, 14 cases of primary colorectal adenocarcinomas, 7 cases of adenocarcinoma metastatic to regional lymph nodes and 7 cases of adenocarcinoma metastatic to distant organs. Each case is sampled three times with 0.6 mm cores.

    This TMA represents a limited number of cases that may detect strong trends in differential gene expression and is intended for pilot surveys and generation of hypotheses. This TMA does not contain case numbers of sufficient quantity to prove the clinical utility of a marker.

    Unstained histologic sections are 4 microns thick on charged glass slides.

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  2. CHTN OvCa2 - Ovarian Carcinoma Survey

    Description:

    The CHTN OvCa2 TMA contains examples of human ovarian carcinoma and ovarian borderline tumors representing the major histologic types of epithelial ovarian tumors. Each case is sampled four times with 0.6 mm cores. The TMA design includes 12 cases each of serous papillary carcinoma, clear cell carcinoma, endometrioid adenocarcinoma and mucinous adenocarcinoma. Another 6 cases each of serous and mucinous borderline tumors are provided. In addition 24 cases of non-malignant tissues (proliferative endometrium, fallopian tube, serous and mucinous cystadenomas) are represented.

    This TMA may detect strong trends in differential gene expression among the different histologic types of ovarian carcinoma, and is intended for pilot surveys and generation of hypotheses. This TMA is not designed to prove the clinical utility of prognostic biomarkers.

    Unstained histologic sections are 4 microns thick on charged glass slides.

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  3. CHTN_BRCAPROG2 - Breast Cancer Progression (Histologic Progression)

    Description:

    The CHTN_BrCaProg2 TMA contains non-neoplastic breast tissue, ductal carcinoma in situ (DCIS), infiltrating ductal carcinoma of the breast, infiltrating lobular carcinoma of the breast, and cases of adenocarcinoma metastatic to regional lymph nodes. Each case is sampled one time with a 2 mm core. The TMA design includes 14 cases of non-neoplastic breast, 14 cases of DCIS, 21 cases of primary carcinoma and 7 cases of metastatic carcinoma. Histologic quality assurance limits ensure a minimum of 7 cases of non-neoplastic breast, 15 cases of infiltrating carcinoma primary to the breast and 4 cases of metatstatic carcinoma. Due to the difficulty in capturing pre-invasive neoplasia, a minimum number of DCIS cases is not being guaranteed.

    This TMA represents a limited number of cases that may detect strong trends in differential gene expression, and is intended for pilot surveys and generation of hypotheses. This TMA does not contain case numbers of sufficient quantity to prove the clinical utility of a marker.

    Unstained histologic sections are 4 microns thick on charged glass slides.

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  4. CHTN_PrC_Prog1 - Prostate Cancer Tumor Progression

    Description:

    The CHTN_PrC_Prog1 TMA contains examples of prostate epithelium representing the stages of tumor progression in prostate adenocarcinoma. Each case is sampled one time with a 1.5 mm core. The TMA design includes 10 cases each of non-neoplastic prostate glands, benign prostate glands from areas of benign prostatic hyperplasia (BPH), high-grade prostatic intraepithelial neoplasia (PIN), prostate adenocarcinoma, Gleason score 5-6, prostate adenocarcinoma, Gleason score 7, and prostate adenocarcinoma, Gleason score 8-10.

    This TMA represents a limited number of cases that may detect strong trends in differential gene expression and is intended for pilot surveys and generation of hypotheses. This TMA does not contain case numbers of sufficient quantity to prove the clinical utility of a marker.

    Unstained histologic sections are 4 microns thick on charged glass slides.

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  5. CHTN_PancProg1 - Pancreas Carcinoma Progression

    Description:

    The CHTN_PancProg1 TMA contains examples of pancreatic invasive carcinoma and intraepithelial neoplasia (PanIN), including high and low- grade pancreatic PanIN and nodal and distant metastases collected from the patients whose primary invasive tumor is also represented. Also included are benign ducts from both benign and malignant pancreas samples. Each case is sampled one time with a 1.5 mm core. There is redundant sampling of most invasive carcinoma cases across for other tissue types, so most invasive carcinomas will be matched to normal, PanIN, or nodal metastasis elsewhere on the array.

    This TMA represents a limited number of cases that may detect strong trends in differential gene expression and is intended for pilot surveys and generation of hypotheses. This TMA does not contain case numbers of sufficient quantity to prove the clinical utility of a marker.

    Unstained histologic sections are 4 microns thick on charged glass slides.

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Cooperative Human Tissue Network (CHTN) Normal and Test Tissue Microarrays

  1. CHTN_NORM2 - Normal Human Tissues

    Description:

    The normal tissue TMA series contains 50 human tissue types in 1.0 mm spot sizes. Most samples are of normal, non-neoplastic adult tissue obtained from surgical resection specimens, excepting parathyroid from a hyperplastic parathyroid gland and central nervous system tissue (cerebral cortex and white matter) from autopsy.

    This TMA is designed provide researchers with a screening tool containing most of the cell types present in the human body.

    Unstained histologic sections are 4 microns thick on charged glass slides.

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  2. CHTNEndo1 - Normal Endometrial Cycle Tissue Microarrays

    Description:

    The CHTN EndoN1 TMA samples normally functioning endometrium through the endometrial cycle. There are 10 cases each of proliferative-phase endometrium, 10 cases each of secretory endometrium at post-ovulatory days 16-18, 19-20, 21-22, 23-24, 25-26 and 7 cases each of secretory endometrium at post-ovulatory days 27-28. Each target tissue type is sampled twice with 1.0 mm cores.

    This TMA is meant to provide an initial survey tool for determining gene expression patterns in endometrial tissue during the menstrual cycle.

    Unstained histologic sections are 4 microns thick on charged glass slides.

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  3. CHTN CNS1 - Human Nervous System

    Description:

    The CHTN CNS1 TMA samples 12 areas of the central nervous system and supporting structures, capturing most of the major cell types of the brain and spinal cord. Each target tissue type is sampled three times with 1.5 mm cores.

    This TMA is meant to provide an initial survey tool for determining gene expression patterns in different cell types of the central nervous system.

    Unstained histologic sections are 4 microns thick on charged glass slides.

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  4. CHTN Test2 - Test Tissue Microarray

    Description:

    CHTN Test Tissue Microarrays (versions 2,3,4) contain lymphoid, epithelial and stromal tissue (spleen, colonic mucosa, prostate, liver, breast cancer and uterine smooth muscle) in 0.6 mm spot sizes. These are useful for determining assay conditions and procedures before using more expensive comprehensiveTMA slides.

    Unstained histologic sections are 4 microns thick on charged glass slides.

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Cancer Diagnosis Program (CDP) Tissue Microarrays

  1. CDP Breast Cancer Progression Tissue Microarray (Clinical Progression)

    Description:

    CDP Breast Cancer Progression Tissue Microarrays are designed to investigate differences in marker prevalence in three stages of invasive breast cancer: node-negative, node-positive and metastatic disease. This TMA set contains a total of 339 breast tissue cores, plus cell line and normal non-breast tissue controls. All of the invasive cases are primary breast cancers with a principal histology of ductal cancer. The CDP breast cancer progression TMA was designed by a National Cancer Institute biostatistician to ensure high statistical power for studies of stage specific markers of breast cancer.

    Unstained histologic sections are 4 microns thick on charged glass slides. 1 set consists of 6 TMA slides.

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  2. CDP Breast Cancer Stage I Prognostic Tissue Microarray

    Description:

    CDP Breast Cancer Stage I Prognostic Tissue Microarrays are designed to examine potential prognostic markers in stage I non-metastatic breast cancer. This TMA set contains a total of 630 breast tissue cores, plus cell line and normal non-breast tissue controls. All of the invasive cases are primary breast cancers with a principal histology of ductal or lobular cancer staged as defined by AJCC Manual for Staging of Cancer, 5th edition. The CDP breast cancer prognostic TMAs were designed by a National Cancer Institute biostatistician to ensure high statistical power.

    Unstained histologic sections are 4 microns thick on charged glass slides. 1 set consists of 10 TMA slides.

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  3. CDP Breast Cancer Stage II Prognostic Tissue Microarray

    Description:

    CDP Breast Cancer Stage II Prognostic Tissue Microarrays are designed to examine potential prognostic markers in stage II non-metastatic breast cancer. This TMA set contains a total of 430 breast tissue cores, plus cell line and normal non-breast tissue controls. All of the invasive cases are primary breast cancers with a principal histology of ductal or lobular cancer staged as defined by AJCC Manual for Staging of Cancer, 5th edition. The CDP breast cancer prognostic TMAs were designed by a National Cancer Institute biostatistician to ensure high statistical power.

    Unstained histologic sections are 4 microns thick on charged glass slides. 1 set consists of 8 TMA slides.

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  4. CDP Breast Cancer Stage III Prognostic Tissue Microarray

    Description:

    CDP Breast Cancer Stage III Prognostic Tissue Microarrays are designed to examine potential prognostic markers in stage III non-metastatic breast cancer. This TMA set contains a total of 197 breast tissue cores, plus cell line and normal non-breast tissue controls. All of the invasive cases are primary breast cancers with a principal histology of ductal or lobular cancer staged as defined by AJCC Manual for Staging of Cancer, 5th edition. The CDP breast cancer prognostic TMAs were designed by a National Cancer Institute biostatistician to ensure high statistical power.

    Unstained histologic sections are 4 microns thick on charged glass slides. 1 set consists of 4 TMA slides.

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  5. CDP Melanoma Progression Tissue Microarray

    Description:

    CDP Melanoma Progression Tissue Microarrays are designed to survey differences in biomarker prevalence in primary, recurrent and metastatic melanoma. This TMA set contains a total of 390 tissue cores from melanoma patients, cell line and normal skin controls, and a test TMA. The CDP melanoma progression TMAs were designed and constructed by a National Cancer Institute grantee, but their statistical power is not stated.

    Unstained histologic sections are 4 microns thick on charged glass slides. 1 set consists of 4 TMA slides.

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